Type 3 Secretion Systems: Methods and Protocols (Methods in Molecular Biology #1531)
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This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and comprehensive, Type 3 Secretion Systems: Methods and Protocols is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.
The book, Type 3 Secretion Systems: Methods and Protocols (Methods in Molecular Biology #1531) [Bulk, Wholesale, Quantity] ISBN#9781493966479 in Hardcover by may be ordered in bulk quantities. Minimum starts at 25 copies. Availability based on publisher status and quantity being ordered.
Details
- Publisher
- Springer New York
- Format
- Hardcover
- Published
- Nov 12, 2016
- ISBN-13
- 9781493966479
- Pages
- 224
- Printing
- Printed on demand
Type 3 Secretion Systems in bulk: pricing
Volume pricing applies automatically in your cart. No codes, no account needed.
| Copies | Price per copy | You save |
|---|---|---|
| 25 – 49 | $96.79 | 12% off list |
| 50 – 99 | $95.69 | 13% off list |
| 100+ | $94.59 | 14% off list |
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